[关键词]
[摘要]
为研究PMA-qPCR方法的适用性问题，本文以超声波制备膜损伤细菌，用PMA-qPCR方法进行检测，并与平板计数的结果进行比较，证明该方法适用于监测超声波法灭菌率的监测。将该方法用于检测不同因素对超声波灭菌效率的影响，表明在温度和超声频率一定的条件下，处理时间和功率对灭菌效率的影响比较明显，而占空比对其影响较小。
[Key word]
[Abstract]
To evaluate the capability of PMA-qPCR method to monitor the sterilization rate of ultrasound, the cultures were treated with ultrasound and then subjected to PMA-qPCR or plate counting. The dates showed that the PMA-qPCR method was quite effective and applicable. Evaluation of the influence of different factors on the sterilization rate showed that the treatment time and the power were the most important parameters at certain temperature and ultrasonic frequency, while the duty ratio showed little influence on the sterilization rate.
[中图分类号]
[基金项目]
国家自然科学基金项目(31101279)；东莞市高等院校科研机构和医疗卫生单位科技计划项目(201010810110)；广东省科技计划项目(2011B020314004)；中央高校基本科研业务费项目(2011ZM0101)；华南理工大学SRP项目
