以白附片为原料，利用酸水法提取，在单因素实验基础上根据Box-Behnken实验设计原理，采用四因素三水平的响应面分析法对总生物碱的提取工艺进行了优化。通过响应面实验，建立了白附片中总生物碱提取率与四个因素变化的二次回归方程。同时依据回归模型进行了计算机模拟实验及绘制曲面图，了解白附片中总生物碱提取率随主要因素水平的变化方向及优化点。结果表明：白附片中总生物碱提取的最佳工艺条件为pH 3.4，液料比11 mL/g，温度30 ℃，时间4.6 h，在此条件下，白附片中总生物碱的提取率为48.5%±1.7%。

Total alkaloid was extracted from white prepared lateral root of Aconite by acidic water method, which was optimized by response surface designs. Based on the results of one-factor test, response surface methodology with 4 factors and 3 levels was adopted according to box-behnken experimental design principle. Two regression equations for the influence relationships of the extraction rate and four varietals factors were established by response surface experiments. On the basis of regression models, the results with surface were simulated to give optimized result and influencing trends of the main factors on the extraction rate. The optimum extracting conditions are as follows: pH 3.4, liquid-to-solid ratio 11 mg/g, extraction time 4.6 h and temperature 30 ℃. Under these conditions, the extraction rate was 48.5%±1.7%.

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