研究应用大孔树脂纯化枸杞叶总黄酮的工艺，比较了AB-8、ADS-5、ADS-7、ADS-17、ADS-21、HP-20六种大孔树脂分离纯化枸杞叶总黄酮的优劣，结果表明，HP-20型大孔树脂对枸杞叶总黄酮的吸附性能与解吸效果最好。确定了其最佳工艺为：上柱液13 BV，上样流速2 mL/min，上样浓度2.336 mg/mL，以70%乙醇为洗脱液，洗脱流速2 mL/min，洗脱剂用量3.5 BV。在此条件纯化后，枸杞叶黄酮提取物中总黄酮含量由29.02%提高到83.75%。该工艺条件科学合理，可有效地用于枸杞叶黄酮的分离富集。高效液相色谱检测芦丁含量由9.88%提高到23.79%。

Purification of total flavones of Lycium Chinese leaves using macroreticular resins was investigated. Dynamic elution ratio of AB-8, ADS-5, ADS-7, ADS-17, ADS-21, and HP-20 were studied respectively and compared in order to find the optimum macroreticular resin. Results indicated that the HP-20 resin was the best due to its highest adsorption and desorption properties. The optimum conditions were: ratio of flavonoid volume to the bed volume of 13 BV, the pH value of sample 6, concentration of sample 2.336 mg/mL, current velocity 2 BV/h, 70% alcoholas eluant for desorption, current velocity 2B V/h and eluant volume 3.5 BV. Under those conditions, and the content of Lycium Chinese leaves flavonoids increased from 29.02% to 83.75%. The technique condition is scientific and reasonable. It can be used to separate and purificate total flavonoids from Lycium Chinese leaves. Flavonoid compounds in leaves of Lycium bararum were analyzed by using HPLC. Lutein content greatly increased from 9.88%to 23.79% after the column purification.

天津市科技成果转化及产业化推进计划项目（09ZHXHNC04900）；福建仙洋洋食品科技有限公司资助项目