以菲牛蛭为原料，采用Alcalase 2.4 L碱性蛋白酶酶解菲牛蛭制备抗凝血肽，通过筛选显著单因素和设计正交试验，并以抗凝血活性为指标，确定酶解的最佳反应条件为：酶解温度55 ℃、酶解时间2 h、底物浓度12%、加酶量3%、pH 8.5，在该条件下所制备的抗凝血肽的抗凝血活性可达702 AT-U/g。

In order to obtain high activity ancoagulant peptide from Poecilobdella manillensis Lesson, an enzymolysis method was established through sequential steps of screening single-factor and designing orthogonal test. By selecting ancoagulant activity as index, the optimum enzymolysis reaction conditions were determined as enzymolysis temperature 55℃, enzymolysis temperature 2h, substrate concentration 12%, enzyme dosage 3% and pH8.5. The ancoagulant activity could reach 702AT-U/g under the optimized conditions.

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