以诱变获得的酿酒酵母突变株YF（ZnCl2r，Ethr）为试验菌株，通过摇瓶发酵、发酵罐补料分批发酵，对突变株发酵生产谷胱甘肽进行研究。确定发酵罐分批发酵的最佳培养条件为：温度30 ℃，pH 6.0，接种量为20%，搅拌转速为150 r/min，通气量为250 L/h。在补料分批操作方式下，分别考察了摇瓶培养、发酵罐培养YF（ZnCl2r，Ethr）对GSH生物合成的影响。确定摇瓶培养条件：初糖10 g/L，发酵4 h、10 h、16 h、22 h、28 h时分别补糖25 g/L、15 g/L、5 g/L、5 g/L、5 g/L使总糖达到65 g/L，测得细胞干重达12.75 g/L，GSH浓度和胞内GSH含量达到76.49 mg/L和0.59%。发酵罐初糖浓度20 g/L，发酵12 h开始补糖24 h使最终总糖达为160 g/L，测得细胞干重为30.95 g/L，GSH浓度和胞内GSH含量达到68.49 mg/L和0.3%。

Fermentation of glutathione was studied through the ways of batch fermentation and fed-batch fermentation by YF（ZnCl2r，Ethr）, a test strain, which is mutant of Saccharomyces cerevisiae. The results show that the optimal fermentation condition: culture temperature 30 ℃, pH 6.0, inoculation 20%，rotate speed 150 r/min，ventilation 250 L/h. The effects of shake-flask culture and fermentor culture on GSH synthesis were studied in fed-batch fermentation. Conditions for the shake-flask culture methods are: initial sugar 10 g/L, fermentation at 4 h, 10 h, 16 h, 22 h, 28 h, respectively, glucose 25 g/L, 15 g/L, 5 g/L, 5 gL, 5 g/L added in fermentation solution, the final total glucose reached 65 g/L, the maximum cell dry weight reached 12.75 g/L, and the maximum GSH concentration and intracellular GSH content reached 76.49 mg/L and 0.59% respectively. The conditions for fermentor culture methods are: initial sugar 20 g/L, 12 h fermentation of sugar begans to fill up to 24 h, the final total glucose reached 160 g/L, the maximum cell dry weight reached 30.95 g/L, the maximum GSH concentration and intracellular GSH content were 68.49 mg/L and 0.3% respectively.